JOURNAL COVER PAGE
 
 
 
 
VOLUME 6, ISSUE 3
July - September 2017
 
Abstract

Comparison of Modified Jaffe’s Kinetic Method and Enzymatic Method of Serum Creatinine Estimation for Precision, Linearity and Effect of Interferent

N. R. Malukar1*, S. B. Kanazariya2, Sunil K Bapure3, Jayendra Purohit4

1Assistant Professor, Biochemistry Department, Medical Collage Baroda. 2Tutor, M.Sc Medical Biochemistry, Shree K. J. Maheta Hospital trust and College of Dental Science, Amargadh, Bhavnagar. 3MDS, Reader Department of Periodontology, Collage of Dental Science, Amargadh, Bhavnagar. 4MDS, Reader Department of Oral And Maxillofacial Surgery, Collage of Dental Science, Amargadh, Bhavnagar.

ABSTRACT

ABSTRACT
BACKGROUND: Serum creatinine (Scr) measurement plays a key role in glomerular filtration rate estimation (eGFR), chronic kidney disease (CKD) diagnosis as well as CKD treatment. The purpose of this study is to find appropriate method of serum creatinine estimation for routine use and in presence of various interferents like glucose and bilirubin.OBJECTIVES: The aim of this paper was to compare precision of serum creatinine estimation by modified Jaffe's kinetic method and enzymatic method. To compare the linearity of serum creatinine estimation by modified Jaffe's kinetic method and enzymatic method. To compare the effects of some common interfering substances like Bilirubin and glucose estimation by modified Jaffe's kinetic method and enzymatic method. MATERIAL AND METHODS: It was an Analytical - Cross sectional Study in a period of 5 months, from February 2016 to June 2016 at Clinical Biochemistry laboratory, S.S.G. Hospital, Baroda. Creatinine was measured by a Modified Jaffe‟s Kinetic method and by an enzymatic method. All tests were done on ROBONIK Semi autoanalyser. RESULTS: Serum creatinine measured by the Modified Jaffe‟s Kinetic and enzymatic methods was statistically significance in healthy subjects (1.02 ± 0.23 vs. 1.10 ± 0.23 mg/dL, respectively, P=0.00001), in diabetic patients (1.30 ± 0.59 vs. 1.17 ± 0.51 mg/dL, respectively, P=0.00001) and jaundice patients (1.33 ± 1.00 vs. 1.67 ± 1.42 mg/dL, respectively, P=0.00001) and in both the parameter bilirubin and glucose was high in the patients (1.41 ± 1.00 vs. 1.46 ± 1.01 mg/dL, respectively, P=0.00433). CONCLUSION: The enzymatic method is more reliable when interfering substances are present in the samples analyzed, which makes a method of choice.

Keywords: Serum Creatinine, Modified Jaffe‟s Kinetic Method, Enzymatic Method, Common Interferent like Bilirubin and Glucose


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